Identification of iron-regulated cellular proteins, Fe3+-reducing and -chelating compounds, in the white-rot fungus Perenniporia medulla-panis.
Can J Microbiol
; 53(12): 1323-9, 2007 Dec.
Article
em En
| MEDLINE
| ID: mdl-18059565
In this paper, we present the responses of the white-rot fungus Perenniporia medulla-panis to iron availability with regard to alterations in growth, expression of cellular proteins, Fe3+-reducing activity, and Fe3+ chelators production. Iron supplementation stimulated fungal growth but did not result in a significant increase in biomass production. Catechol and hydroxamate derivatives were produced mainly under iron deficiency, and their productions were repressed under iron supplementation conditions. Perenniporia medulla-panis showed several cellular proteins in the range of 10-90 kDa. Some of them showed negative iron-regulation. Iron-supplemented medium also repressed both cell surface and extracellular Fe3+-reducing activities; however, the highest cell surface activity was detected at the initial growth phase, whereas extracellular activity increased throughout the incubation period. No significant production of chelators and extracellular Fe3+-reducing activity were observed within the initial growth phase, suggesting that the reduction of Fe3+ to Fe2+ is performed by ferrireductases.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polyporaceae
/
Compostos Férricos
/
Ferro
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Can J Microbiol
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Canadá