Extracellular matrix gene expression by human endothelial and smooth muscle cells.
Matrix
; 11(6): 380-7, 1991 Dec.
Article
em En
| MEDLINE
| ID: mdl-1787830
In this study, the expression of extracellular matrix genes by vascular cells from human iliac blood vessels was characterized on the mRNA steady-state level by slot blot and Northern transfer analyses, as well as by in situ hybridization. Endothelial cells were isolated from adult human iliac arteries and veins, as well as from umbilical veins; smooth muscle cells were isolated from adult human iliac arteries and inferior vena cava. The results show that confluent umbilical vein endothelial cells expressed the genes that encode types I, III, IV and VI collagens, as well as fibronectin and laminin. In contrast, the iliac endothelial cells expressed the genes for types IV and V collagens, fibronectin and laminin; mRNA transcripts for types I, III and VI collagens were not detectable. The smooth muscle cells from iliac arteries or inferior vena cava displayed gene expression for types I, III, IV, V and VI collagens, fibronectin and laminin. The results indicate major differences in gene expression for the various types of collagens by human iliac endothelial and smooth muscle cells. Furthermore, the fetal-derived umbilical endothelial cells displayed differential collagen gene expression from that of adult iliac endothelial cells.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Endotélio Vascular
/
Proteínas da Matriz Extracelular
/
Músculo Liso Vascular
Limite:
Adolescent
/
Adult
/
Humans
Idioma:
En
Revista:
Matrix
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
1991
Tipo de documento:
Article
País de publicação:
Alemanha