Imipenem resistance associated with the loss of a 40 kDa outer membrane protein in Enterobacter aerogenes.
J Antimicrob Chemother
; 28(4): 499-504, 1991 Oct.
Article
em En
| MEDLINE
| ID: mdl-1761444
An imipenem-resistant strain, Enterobacter aerogenes EA-Z, was isolated from a blood culture. Outer membrane protein (OMP) profiles indicated the loss of a 40 kDa OMP, decreased expression of 42 and 44 kDa OMPs, and increased expression of a 50 kDa OMP in strain EA-Z when compared with imipenem-susceptible clinical isolates of E. aerogenes. The OMP profile of EA-Z was similar to that of strain EA-SI16, an imipenem-resistant E. aerogenes second-step mutant selected on imipenem-containing media. A single-step imipenem-resistant mutant, EA-SI8, had lost expression of only the 40 kDa OMP. No new beta-lactamase could be detected by isoelectric focusing, and no increase in imipenem hydrolysis was seen when EA-Z was compared with imipenem-sensitive controls, even in the presence of added zinc. These data suggest that the 40 kDa OMP of E. aerogenes might be required for the normal diffusion of imipenem across the outer membrane.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas da Membrana Bacteriana Externa
/
Imipenem
/
Klebsiella pneumoniae
Tipo de estudo:
Risk_factors_studies
Idioma:
En
Revista:
J Antimicrob Chemother
Ano de publicação:
1991
Tipo de documento:
Article
País de publicação:
Reino Unido