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Molecular screening shows extensive HIV-1 genetic diversity in Central West Brazil.
Stefani, Mariane M A; Pereira, Gisner A S; Lins, Janaina A B; Alcantara, Keila C; Silveira, Alexsander A; Viegas, Angela A; Maya, Nadia C; Mussi, Aparecida Hg.
Afiliação
  • Stefani MM; Tropical Pathology and Public Health Institute, Federal University of Goias, Rua Delenda Rezende de Melo s/n, Setor Universitario, 74.605-050 Goiania, Goias, Brazil. mstefani@iptsp.ufg.br
J Clin Virol ; 39(3): 205-9, 2007 Jul.
Article em En | MEDLINE | ID: mdl-17537671
BACKGROUND: Information is limited about HIV-1 subtypes circulating in less populated Brazilian areas where the AIDS epidemic is expanding, such as in the Central West region. OBJECTIVE: To describe HIV-1 subtypes in patients from the States of Goias, Mato Grosso do Sul and Mato Grosso in Central West Brazil. STUDY DESIGN: Heteroduplex mobility analysis of nested-PCR products from env (primers: ED5/ED12, ES7/ES8) and gag regions (primers: H1P202/H1G777, H1Gag1584/g17) of 406 HIV-1 isolates from Goias (n=271), Mato Grosso do Sul (n=85) and Mato Grosso (n=50) collected from 2001 to 2004. RESULTS: Median age of patients was 26 years (1-79 range), 68.7% (279/406) females, 69.9% (269/385) sexual exposure, 14.3% parenteral risk, 15.8% vertical cases. Overall 69.9% (284/406) of HIV-1 subtypes were concordant B(env)/B(gag), 1.7% F(env)/F(gag) and 1% C(env)/C(gag). Discordant HIV-1 isolates were 14.5% (59/406), mainly B(env)/F(gag) and F(env)/B(gag) (49/59); five were B(env)/D(gag), four B(env)/C(gag) and one C(env)/D(gag). B/B and discordant B/F isolates were detected among all risk categories and among children and adults. CONCLUSION: Extensive genetic diversity of HIV-1 was observed in Central West Brazil. Continued molecular studies should monitor the changing dynamics of HIV-1 over time especially in areas where the epidemic is growing.
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Variação Genética / Infecções por HIV / HIV-1 / Epidemiologia Molecular Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Adolescent / Adult / Aged / Child / Child, preschool / Female / Humans / Infant / Male / Middle aged País/Região como assunto: America do sul / Brasil Idioma: En Revista: J Clin Virol Assunto da revista: VIROLOGIA Ano de publicação: 2007 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
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Adicionar na Minha BVS
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Variação Genética / Infecções por HIV / HIV-1 / Epidemiologia Molecular Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Adolescent / Adult / Aged / Child / Child, preschool / Female / Humans / Infant / Male / Middle aged País/Região como assunto: America do sul / Brasil Idioma: En Revista: J Clin Virol Assunto da revista: VIROLOGIA Ano de publicação: 2007 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda