Identification of type 1 and type 2 light-harvesting chlorophyll a/b-binding proteins using monospecific antibodies.
Biochim Biophys Acta
; 1098(2): 191-200, 1992 Jan 16.
Article
em En
| MEDLINE
| ID: mdl-1730008
The amino acid sequences of more than 40 apoproteins of the light-harvesting complex associated with Photosystem II (LHC II) of various plants have been deduced by sequencing their corresponding genes. These highly conserved sequences fall into two major categories, type 1 and type 2, that differ mainly in a small number of domains close to the N-terminus. We have made polyclonal, monospecific antibodies against synthetic peptides corresponding to the most unique sequence domains of the N-terminal regions of type 1 and type 2 LHC II apoproteins, using sequences derived from petunia genes. On Western blots our anti-type 1 and 2 antibodies crossreact with light-harvesting proteins of petunia, tomato, spinach and several other plants. By using a new gel-system based on ammediol (2-amino-2-methyl-1,3-propanediol), we are able to resolve up to eight LHC II apoproteins. On petunia, tomato and spinach blots the anti type 1 antibodies bind to two or more of the higher molecular weight LHC II polypeptides, whereas the anti type 2 antibodies recognize very specifically only one or two of the lower molecular weight LHC-proteins. In all plants studied, the type 1 LHC II apoproteins are more numerous and span a greater size range than the type 2 apoproteins. This is consistent with the smaller number of type 2 LHC II CAB genes that have been discovered to date.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Apoproteínas
/
Proteínas de Plantas
/
Complexo de Proteínas do Centro de Reação Fotossintética
/
Complexos de Proteínas Captadores de Luz
/
Complexo de Proteína do Fotossistema II
/
Anticorpos
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
1992
Tipo de documento:
Article
País de publicação:
Holanda