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The mycobiota and toxicity of equine feeds.
Keller, K M; Queiroz, B D; Keller, L A M; Ribeiro, J M M; Cavaglieri, L R; Pereyra, M L González; Dalcero, A M; Rosa, C A R.
Afiliação
  • Keller KM; Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Instituto de Veterinária, Rio de Janeiro, Brazil.
Vet Res Commun ; 31(8): 1037-45, 2007 Nov.
Article em En | MEDLINE | ID: mdl-17285246
Feed contamination can lead to nutrient losses and detrimental effects on animal health and production. The purposes of this study were to investigate the mycobiota in equine mixed feeds and to determine natural contamination with aflatoxin B1 (AFB1) and fumonisin B1 (FB1). Fungal enumeration of equine feed samples was done. A commercially available enzyme-linked immunosorbent assay kit was applied to quantify AFB1 and FB1. A comparison between ELISA and HPLC was carried out. Feed mould counts ranged from <1 x 10(2) to 1 x 10(5) cfu/g. The most frequent genus isolated was Aspergillus (40.54%), followed by Penicillium (18.38%) and Fusarium (16.22%). The most prevalent Aspergillus sp. was A. flavus (36%). AFB1 values ranged between 0.01 and 99.4 microg/kg. FB(1) levels ranged between 0.01 and 7.49 microg/kg. HPLC and ELISA methods showed positive correlation for AFB1 and FB1 determinations (r = 0.9851 and r = 0.9791, respectively). The ELISA analytical method was efficient for AFB1 and FB1 detection. The scarcity of studies on natural fungal contamination and on the presence of AFB1 and FB1 in materials used as equine feed ingredients highlights the value and contribution of this study.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aflatoxina B1 / Fumonisinas / Doenças dos Cavalos / Ração Animal Limite: Animals Idioma: En Revista: Vet Res Commun Ano de publicação: 2007 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aflatoxina B1 / Fumonisinas / Doenças dos Cavalos / Ração Animal Limite: Animals Idioma: En Revista: Vet Res Commun Ano de publicação: 2007 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça