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Chromatin remodeling by SWI/SNF results in nucleosome mobilization to preferential positions in the rat osteocalcin gene promoter.
Gutiérrez, José; Paredes, Roberto; Cruzat, Fernando; Hill, David A; van Wijnen, Andre J; Lian, Jane B; Stein, Gary S; Stein, Janet L; Imbalzano, Anthony N; Montecino, Martin.
Afiliação
  • Gutiérrez J; Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias Biologicas, Universidad de Concepcion, Barrio Universitario s/n, Casilla 160-C, Concepcion 4079100, Chile.
  • Paredes R; Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias Biologicas, Universidad de Concepcion, Barrio Universitario s/n, Casilla 160-C, Concepcion 4079100, Chile.
  • Cruzat F; Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias Biologicas, Universidad de Concepcion, Barrio Universitario s/n, Casilla 160-C, Concepcion 4079100, Chile.
  • Hill DA; Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655.
  • van Wijnen AJ; Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655.
  • Lian JB; Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655.
  • Stein GS; Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655.
  • Stein JL; Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655.
  • Imbalzano AN; Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655.
  • Montecino M; Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias Biologicas, Universidad de Concepcion, Barrio Universitario s/n, Casilla 160-C, Concepcion 4079100, Chile. Electronic address: mmonteci@udec.cl.
J Biol Chem ; 282(13): 9445-9457, 2007 Mar 30.
Article em En | MEDLINE | ID: mdl-17272279
Changes in local chromatin structure accompany transcriptional activation of eukaryotic genes. In vivo these changes in chromatin organization can be catalyzed by ATP-dependent chromatin-remodeling complexes, such as SWI/SNF. These complexes alter the tight wrapping of DNA in the nucleosomes and can facilitate the mobilization of the histone octamer to adjacent DNA segments, leaving promoter regulatory elements exposed for transcription factor binding. To gain understanding of how the activity of SWI/SNF complexes may be modulated by the different DNA sequences within a natural promoter, we have reconstituted nucleosomes containing promoter segments of the transcriptionally active cell type-specific osteocalcin (OC) gene and determined how they affect the directional movements of the nucleosomes. Our results indicate that SWI/SNF complexes induce octamer sliding to preferential positions in the OC promoter, leading to a nucleosomal organization that resembles that described in intact cells expressing the OC gene. Our studies demonstrate that the position of the histone octamer is primarily determined by sequences within the OC promoter that include or exclude nucleosomes. We propose that these sequences are critical components of the regulatory mechanisms that mediate expression of this tissue-specific gene.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Proteínas Cromossômicas não Histona / Nucleossomos / Osteocalcina / Regiões Promotoras Genéticas / Montagem e Desmontagem da Cromatina Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: J Biol Chem Ano de publicação: 2007 Tipo de documento: Article País de afiliação: Chile País de publicação: Estados Unidos
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Proteínas Cromossômicas não Histona / Nucleossomos / Osteocalcina / Regiões Promotoras Genéticas / Montagem e Desmontagem da Cromatina Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: J Biol Chem Ano de publicação: 2007 Tipo de documento: Article País de afiliação: Chile País de publicação: Estados Unidos