Early gene expression during natural spinal cord regeneration in the salamander Ambystoma mexicanum.
J Neurochem
; 101(1): 27-40, 2007 Apr.
Article
em En
| MEDLINE
| ID: mdl-17241119
In contrast to mammals, salamanders have a remarkable ability to regenerate their spinal cord and recover full movement and function after tail amputation. To identify genes that may be associated with this greater regenerative ability, we designed an oligonucleotide microarray and profiled early gene expression during natural spinal cord regeneration in Ambystoma mexicanum. We sampled tissue at five early time points after tail amputation and identified genes that registered significant changes in mRNA abundance during the first 7 days of regeneration. A list of 1036 statistically significant genes was identified. Additional statistical and fold change criteria were applied to identify a smaller list of 360 genes that were used to describe predominant expression patterns and gene functions. Our results show that a diverse injury response is activated in concert with extracellular matrix remodeling mechanisms during the early acute phase of natural spinal cord regeneration. We also report gene expression similarities and differences between our study and studies that have profiled gene expression after spinal cord injury in rat. Our study illustrates the utility of a salamander model for identifying genes and gene functions that may enhance regenerative ability in mammals.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Medula Espinal
/
Traumatismos da Medula Espinal
/
Regulação da Expressão Gênica
/
Ambystoma
/
Regeneração Nervosa
/
Plasticidade Neuronal
Tipo de estudo:
Prognostic_studies
Limite:
Animals
País/Região como assunto:
Mexico
Idioma:
En
Revista:
J Neurochem
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Estados Unidos
País de publicação:
Reino Unido