[Rapid identification of non tuberculous mycobacteria by restriction pattern analysis]. / Identificación rápida de micobacterias no tuberculosas mediante análisis de patrones de restricción.
Rev Med Chil
; 134(7): 868-73, 2006 Jul.
Article
em Es
| MEDLINE
| ID: mdl-17130970
BACKGROUND: The frequency of diseases caused by non tuberculous mycobacteria has increased in the last years. Their clinical diagnosis is difficult, mainly in immunocompromised patients. The identification of these mycobacteria by traditional methods is based on phenotypic characteristics and the results are obtained two to four weeks after their isolation in primary cultures. AIM: To report a new identification method for non tuberculous mycobacteria. MATERIAL AND METHODS: The restriction pattern analysis method was implemented. It is based on the amplification, using polymerase chain reaction (PCR), of a polymorphic region of 440 base pairs that codifies Hsp65 protein, followed by a digestion with BstE II and Hae III restriction enzymes. The results were compared with patterns established for each strain. RESULTS: Sixty four strains of mycobacteria obtained from clinical samples and seven reference mycobacteria, were identified using the traditional methods and restriction pattern analysis. The latter method identified the same strain as the former in 87.5% of cases. In the remainder 12.5% of cases there was no agreement between both methods. In these, the sequencing of a fragment of a gene that codifies 16S ribosomal RNA, confirmed the correct identification by restriction patterns. CONCLUSIONS: Restriction pattern analysis is a rapid identification method for non tuberculous mycobacterial strains.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Mapeamento por Restrição
/
Reação em Cadeia da Polimerase
/
Técnicas de Tipagem Bacteriana
/
Micobactérias não Tuberculosas
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Idioma:
Es
Revista:
Rev Med Chil
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Chile
País de publicação:
Chile