Monoclonal antibodies to conserved regions of the major core protein (gag24) of HIV-1 and HIV-2.
AIDS Res Hum Retroviruses
; 7(1): 97-101, 1991 Jan.
Article
em En
| MEDLINE
| ID: mdl-1707645
Five mouse monoclonal antibodies were raised against a recombinant protein comprising the complete sequence of gag24 protein from HTLV-IIIB. All monoclonal antibodies recognized the native protein in enzyme-linked immunosorbant assay (ELISA) and Western blots. All monoclonal antibodies also cross-reacted with an human immunodeficiency virus type 2 (HIV-2) strain in western blots, whereas only one antibody detected HIV-2 p25 in ELISA. By using synthetic peptides, cross-reacting epitopes were mapped and three regions were defined. The conserved immunogenic sites were located in the carboxyterminal region of the protein. Inhibition experiments with human sera showed that this region is also immunogenic in humans.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Antígenos HIV
/
Produtos do Gene gag
/
Proteínas do Core Viral
/
HIV-1
/
HIV-2
/
Anticorpos Monoclonais
/
Anticorpos Antivirais
Limite:
Animals
/
Humans
Idioma:
En
Revista:
AIDS Res Hum Retroviruses
Assunto da revista:
SINDROME DA IMUNODEFICIENCIA ADQUIRIDA (AIDS)
Ano de publicação:
1991
Tipo de documento:
Article
País de afiliação:
Cuba
País de publicação:
Estados Unidos