Biosurfactant production by Rhodococcus erythropolis grown on glycerol as sole carbon source.

Ciapina, Elisa M P; Melo, Walber C; Santa Anna, Lidia M M; Santos, Alexandre S; Freire, Denise M G; Pereira Júnior, Nei

Ciapina, Elisa M P; Melo, Walber C; Santa Anna, Lidia M M; Santos, Alexandre S; Freire, Denise M G; Pereira Júnior, Nei.

Afiliação

Ciapina EM; Departamento de Bioquímica, Universidade Federal do Rio de Janeiro, Centro de Tecnologia, Bloco E, Rio de Janeiro, RJ, CEP 21949-900, Brasil.

Appl Biochem Biotechnol ; 129-132: 880-6, 2006.

Article em En | MEDLINE | ID: mdl-16915696

RESUMO

The production of biosurfactant by Rhodococcus erythropolis during the growth on glycerol was investigated. The process was carried out at 28 degrees C in a 1.5-L bioreactor using glycerol as carbon source. The bioprocess was monitored through measurements of biosurfactant concentration and glycerol consumption. After 51 h of cultivation, 1.7 g/L of biosurfactant, surface, and interfacial tensions values (with n-hexadecane) of 43 and 15 mN/m, respectively, 67% of Emulsifying Index (E24), and 94% of oil removal were obtained. The use of glycerol rather than what happens with hydrophobic carbon source allowed the release of the biosurfactant, originally associated to the cell wall.

Assuntos

Reatores Biológicos/microbiologia; Carbono/metabolismo; Técnicas de Cultura de Células/métodos; Glicerol/metabolismo; Rhodococcus/metabolismo; Tensoativos/química; Tensoativos/metabolismo; Esgotos/microbiologia; Tensoativos/análise

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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tensoativos / Rhodococcus / Carbono / Técnicas de Cultura de Células / Reatores Biológicos / Glicerol Idioma: En Revista: Appl Biochem Biotechnol Ano de publicação: 2006 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos

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