Mycobacterium bovis cultured from commercially pasteurized cows' milk: laboratory cross-contamination.
Vet Microbiol
; 116(4): 325-8, 2006 Sep 10.
Article
em En
| MEDLINE
| ID: mdl-16766143
The ability of Mycobacterium paratuberculosis to survive the commercial pasteurization process of raw milk remains controversial. In a study undertaken in Venezuela to culture M. paratuberculosis from commercially pasteurized cows' milk, 83-200 ml containers of milk were processed and cultured on Herrold's egg yolk slants. No M. paratuberculosis was cultured but a total of six colonies of Mycobacterium bovis were isolated from one container each from two different milk providers. Because laboratory cross-contamination was suspected, the laboratory records were reviewed and spoligotyping was carried out on the isolated individual colonies. On the day before these milk specimens were processed, the biological safety cabinet had been used for the isolation of M. bovis from lymph nodes from infected cattle. Spoligotyping showed that that the colonies isolated from the milk all had the same pattern as the strains isolated from the lymph nodes that were processed the previous day. As far as we know, this is the first report of cross-contamination in a veterinary mycobacterial laboratory. False-positive cultures in the mycobacterial laboratory are not rare. In this setting M. bovis was isolated because it is the most common manipulated organism in this laboratory. We believe that reports on the isolation of M. paratuberculosis from commercially pasteurized milk should exclude cross-contamination before reporting, especially when this organism is routinely isolated from animal material in the same lab.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Contaminação de Alimentos
/
Técnicas de Tipagem Bacteriana
/
Técnicas de Laboratório Clínico
/
Leite
/
Mycobacterium bovis
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Vet Microbiol
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Venezuela
País de publicação:
Holanda