Fluorescent staining as a method of assessing membrane damage and post-thaw survival of ram spermatozoa.
Theriogenology
; 41(2): 483-9, 1994 Feb.
Article
em En
| MEDLINE
| ID: mdl-16727406
We studied the relationship between motility and membrane damage, as assessed by fluorescent staining, in fresh and in frozen-thawed ram spermatozoa. Semen from Merino rams was incubated with 6-carboxyfluorescein diacetate and propidium iodide. In both fresh and frozen-thawed samples, the percentage of intact spermatozoa was lower than the motility rate, thus indicating the presence of damaged but motile spermatozoa. Freezing and thawing resulted in a marked loss of membrane integrity, whereas motility decreased to a lesser extent. There was a positive relationship (r=0.64; P<0.001) between membrane integrity immediately after thawing and motility after 8 h of incubation at 37 degrees C. These results demonstrate the usefulness of the fluorescent staining method for the prediction of ram sperm quality and post-thaw survival.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Theriogenology
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Argentina
País de publicação:
Estados Unidos