Production of beta-galactosidase by Trichoderma reesei FTKO-39 in wheat bran: partial purification of two isozymes.

Adalberto, P R; Massabni, A C; Goulart, A J; Contiero, J; Carmona, E C; Cardello, L; Monti, R

Adalberto, P R; Massabni, A C; Goulart, A J; Contiero, J; Carmona, E C; Cardello, L; Monti, R.

Afiliação

Adalberto PR; Department of General Inorganic and Chemistry, Institute of Chemistry, Rua Prof. Francisco Degni, s/n, 14800-900, Araraquara, SP, Brazil.

Appl Biochem Biotechnol ; 133(2): 163-70, 2006 May.

Article em En | MEDLINE | ID: mdl-16702608

RESUMO

Trichoderma reesei FTKO-39 grown at 35 degrees C for 5 d on wheat bran supplemented with MgCl2 and lactose as the carbon source produced two isozymes of beta-galactosidase: BGT I and BGT II. These isozymes were partially purified on a DEAE-Trisacryl column. Both BGT I and BGT II fractions exhibited optimum activity at 65 degrees C, but the pH optima were 4.0 and 6.5, respectively. The isozymes also showed similar thermal stability. However, BGT I was more stable than BGT II in a pH range of 3.0-10.0. At least two different beta-galactosidases are produced by T. reesei, as revealed by the two bands seen on a 6% polyacrylamide gel stained for activity.

Assuntos

Fibras na Dieta/metabolismo; Fibras na Dieta/microbiologia; Trichoderma/enzimologia; Triticum/microbiologia; beta-Galactosidase/biossíntese; beta-Galactosidase/isolamento & purificação; Isoenzimas/biossíntese; Isoenzimas/química; Isoenzimas/isolamento & purificação; Cinética; Triticum/enzimologia; beta-Galactosidase/química

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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Trichoderma / Triticum / Fibras na Dieta / Beta-Galactosidase Idioma: En Revista: Appl Biochem Biotechnol Ano de publicação: 2006 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos

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