In vitro and in vivo inhibition of skin matrix metalloproteinases by Pothomorphe umbellata root extract.
Photochem Photobiol
; 82(2): 439-42, 2006.
Article
em En
| MEDLINE
| ID: mdl-16613496
Exposure to UV radiation up-regulates the synthesis of matrix metalloproteinases (MMPs), a group of matrix-degrading enzymes. MMPs are regarded as promising therapeutic targets and the development of effective inhibitors is an important research focus. The plant Pothomorphe umbellata has been shown to exert a potent antioxidant activity on the skin and to delay the onset and reduce the incidence of UVB-induced chronic skin damage. The aim of the present study was to determine the effect of P. umbellata ethanolic root extract on MMP-2 and MMP-9. The in vitro inhibition of MMP-2 and MMP-9 was measured by gelatin zymography in the presence of different concentrations of P. umbellata extract, as well as in the presence of its isolated active principle 4-nerolidylcatechol (4-NC). The inhibitory effect of the P. umbellata extract was stronger than that of 4-NC. Gelatin zymography and histological analysis revealed that P. umbellata was able to inhibit constitutive MMP-9 activity in vivo in mice sacrificed 2 h after UVB irradiation. The intensity of the MMP-2 band was unchanged. Our data contribute to the elucidation of the mechanism of prevention of photoaging by P. umbellata and may provide a rational basis for the use of this plant in prophylaxis against and treatment of skin cancer.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Protetores contra Radiação
/
Pele
/
Extratos Vegetais
/
Raízes de Plantas
/
Piperaceae
/
Inibidores de Metaloproteinases de Matriz
Limite:
Animals
Idioma:
En
Revista:
Photochem Photobiol
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Estados Unidos