Improved pharmacological properties for superoxide dismutase modified with mannan.
Biotechnol Appl Biochem
; 44(Pt 3): 159-65, 2006 Jun.
Article
em En
| MEDLINE
| ID: mdl-16556127
Mannan from Sacharomyces cerevisiae was activated by oxidation with NaIO(4) (sodium m-periodate) and further linked to SOD (superoxide dismutase) via reductive alkylation with NaBH(4) (sodium borohydride). The glycosidated enzyme contained an average of 1.2 mol of polysaccharide per mol of protein and retained 52% of its initial activity. The modified enzyme was 560-fold more resistant to inactivation with H(2)O(2) and acquired a lectin-recognition capacity in respect of concanavalin A. The anti-inflammatory activity of SOD was increased 2-fold and its plasma half-life time was prolonged from 4.8 min to 1.7 h after glycosylation with the polymer.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Superóxido Dismutase
/
Inflamação
/
Mananas
Limite:
Animals
Idioma:
En
Revista:
Biotechnol Appl Biochem
Assunto da revista:
BIOQUIMICA
/
BIOTECNOLOGIA
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Cuba
País de publicação:
Estados Unidos