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Identification of an immunogenic protein of Actinobacillus seminis that is present in microvesicles.
Núñez-del Arco, Alma; Salas-Téllez, Enrique; de la Garza, Mireya; Díaz-Aparicio, Efrén; Tenorio-Gutiérrez, Víctor.
Afiliação
  • Núñez-del Arco A; Cátedra de Inmunodiagnóstico de Enfermedades Bacterianas y Micóticas, Facultad de Estudios Superiores (FES) Cuautitlán, Universidad Nacional Autónoma de Mexico (UNAM), Av. Primero de mayo s/n, Cuautitlân Izcalli, Estado de Mexico 54740, Mexico.
Can J Vet Res ; 70(1): 43-9, 2006 Jan.
Article em En | MEDLINE | ID: mdl-16548331
Actinobacillus seminis is a gram-negative bacterium of the Pasteurellaceae family that is involved in ovine epididymitis. Looking for a protein specific to this species, we determined the protein profile of subcellular fractions of A. seminis (American Type Culture Collection number 15768): proteins from the outer membrane (OMPs), inner membrane (IMPs), and cytoplasm (CPs). These profiles provide the first data, to our knowledge, regarding subcellular fractions of A. seminis. In the OMP fraction, we identified a protein with a molecular mass of 75 kDa that proved to be immunogenic and apparently specific for A. seminis. This conclusion was based on the reaction of hyperimmune serum of rabbits inoculated with whole cells of A. seminis that was tested against sonicated complete cells of reference strains and field isolates of Brucella ovis, Mannheimia haemolytica, Pasteurella multocida, and Histophilus somni. No protein of these bacteria cross-reacted with the 75-kDa protein of A. seminis. Furthermore, when each type of hyperimmune serum was tested against the sonicated cells and each of the subcellular fractions of A. seminis, it did not recognize the A. seminis 75-kDa protein. We also isolated and identified this protein in microvesicles released to the culture supernatant. The results suggest that the 75-kDa protein could be used to establish a diagnostic test specific for ovine epididymitis caused by A. seminis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças dos Ovinos / Proteínas de Bactérias / Infecções por Actinobacillus / Actinobacillus seminis / Epididimite / Proteínas de Membrana Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals Idioma: En Revista: Can J Vet Res Assunto da revista: MEDICINA VETERINARIA Ano de publicação: 2006 Tipo de documento: Article País de afiliação: México País de publicação: Canadá
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças dos Ovinos / Proteínas de Bactérias / Infecções por Actinobacillus / Actinobacillus seminis / Epididimite / Proteínas de Membrana Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals Idioma: En Revista: Can J Vet Res Assunto da revista: MEDICINA VETERINARIA Ano de publicação: 2006 Tipo de documento: Article País de afiliação: México País de publicação: Canadá