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Performance of the serologic and molecular screening of blood donations for the hepatitis B and C viruses in a Mexican Transfusion Center.
Chiquete, Erwin; Sánchez, Laura V; Becerra, Guadalupe; Quintero, Angeles; Maldonado, Montserrat; Panduro, Arturo.
Afiliação
  • Chiquete E; Department of Molecular Biology in Medicine, Hospital Civil de Guadalajara Fray Antonio Alcalde, and Department of Physiology, CUCS, Universidad de Guadalajara, Jalisco, México.
Ann Hepatol ; 4(4): 275-8, 2005.
Article em En | MEDLINE | ID: mdl-16432494
Nucleic acid-amplification testing (NAT) is not routinely practiced in blood banks from most low-income countries. We did an exploratory comparison of the performance of the standard immunoassay-based screening tests for the hepatitis B (HBV) and C (HCV) viruses with that of NAT, in blood donors. From January 1999 to March 2005, 94,806 blood donors were screened for anti-HCV antibodies and for hepatitis B surface antigen (HBsAg). Also, an exploratory period of molecular screening was carried out on 100 consecutive blood donors to detect HBV DNA and HCV RNA by home-made PCR techniques without sera pooling. In the 75-month period of serologic screening, HBsAg was detected in 219 donors (0.23%; 95% CI, 0.20- 0.26%) and anti-HCV antibodies in 922 (0.97%; 95% CI, 0.90-1.03%). The annual trend for HBsAg prevalence had a decreasing pattern over the years (p<0.001), whereas that for anti-HCV did not (p=0.19). In the molecular screening cohort, HBV DNA was detected in one donor (1%; 95% CI, 0-6%) and HCV RNA in another (1%; 95% CI, 0-6%). All these 100 donors tested negative to HBsAg and anti-HCV. Thus, the prevalence of positive results for HBV and HCV did not differ if considering immunoassays or NAT; nevertheless, these methods did not coincide in detecting HBV or HCV in the molecular screening cohort. In conclusion, NAT can detect cases of HBV and HCV infections that standard immunoassay techniques can not, even in a highly selected population at low risk, like blood donors. Large-scale studies are warranted for NAT to be considered as a systematic method for screening of HBV and HCV in Mexican blood banks.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bancos de Sangue / Doadores de Sangue / Vírus da Hepatite B / Hepatite C / Hepacivirus / Hepatite B Tipo de estudo: Diagnostic_studies / Risk_factors_studies / Screening_studies Limite: Adolescent / Adult / Aged / Humans / Middle aged País/Região como assunto: Mexico Idioma: En Revista: Ann Hepatol Assunto da revista: GASTROENTEROLOGIA Ano de publicação: 2005 Tipo de documento: Article País de publicação: México
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bancos de Sangue / Doadores de Sangue / Vírus da Hepatite B / Hepatite C / Hepacivirus / Hepatite B Tipo de estudo: Diagnostic_studies / Risk_factors_studies / Screening_studies Limite: Adolescent / Adult / Aged / Humans / Middle aged País/Região como assunto: Mexico Idioma: En Revista: Ann Hepatol Assunto da revista: GASTROENTEROLOGIA Ano de publicação: 2005 Tipo de documento: Article País de publicação: México