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DNA oxidative damage in patients with dialysis treatment.
Domenici, Fernanda Aparecida; Vannucchi, Maria T I; Jordão, Alceu A; Meirelles, Mônica S S; Vannucchi, Helio.
Afiliação
  • Domenici FA; Nutrition Division, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Brazil.
Ren Fail ; 27(6): 689-94, 2005.
Article em En | MEDLINE | ID: mdl-16350819
BACKGROUND/AIMS: Chronic renal patients on hemodialysis (HD) and peritoneal dialysis (PD) treatment are exposed to oxidative stress and DNA damage. The objective of this study was to assess the oxidative damage to DNA in end-stage chronic renal failure, before and after vitamin E supplementation. METHODS: Patients on HD (n=29) and PD (n=22) received oral supplementation with 300 mg vitamin E three times a week for 4 weeks. A blood sample was collected at the beginning and at the end of the supplementation cycle for the determination of vitamin E levels (high-performance liquid chromatography), carbonyl groups, and DNA damage (8-hydroxy 2'-deoxyguanosine [8-OHdG] and comet assay). RESULTS: After supplementation, vitamin E concentration was increased by about 50%. Protein oxidation was initially observed in both groups, with a reduction after supplementation. DNA damage detected by the comet assay and by 8-OHdG analysis was significantly reduced (p<0.05) after supplementation in both groups. CONCLUSIONS: Vitamin E supplementation reduced oxidative DNA damage in both HD and PD patients. Treatments such as HD and PD induce oxidative stress and consequent DNA damage, and increased plasma vitamin E levels significantly contribute to the normalization of these events.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vitamina E / Diálise Renal / Diálise Peritoneal Ambulatorial Contínua / Estresse Oxidativo / Antioxidantes Tipo de estudo: Diagnostic_studies / Etiology_studies / Incidence_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Adult / Female / Humans / Male / Middle aged País/Região como assunto: America do sul / Brasil Idioma: En Revista: Ren Fail Assunto da revista: NEFROLOGIA Ano de publicação: 2005 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vitamina E / Diálise Renal / Diálise Peritoneal Ambulatorial Contínua / Estresse Oxidativo / Antioxidantes Tipo de estudo: Diagnostic_studies / Etiology_studies / Incidence_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Adult / Female / Humans / Male / Middle aged País/Região como assunto: America do sul / Brasil Idioma: En Revista: Ren Fail Assunto da revista: NEFROLOGIA Ano de publicação: 2005 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido