Measurement of melatonin and its metabolites: importance for the evaluation of their biological roles.
Endocrine
; 27(2): 111-8, 2005 Jul.
Article
em En
| MEDLINE
| ID: mdl-16217124
Many physiologic changes related to light-dark cycles and antioxidant effects have been related to melatonin (N-acetyl-5-methoxytryptamine) and its metabolites, N1-acetyl-N2-formyl-5-methoxykynuramine (AFMK) and N1-acetyl-5-methoxykynuramine (AMK). In this review, we discuss some methodologies, in particular, those employing high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) assays to quantitatively determine melatonin, AFMK, and AMK. These approaches offer a highly specific and an accurate quantification of melatonin and its metabolites. These characteristics are essential to point out correctly the biological effects of these compounds in physiological and pathological conditions.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Melatonina
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Endocrine
Assunto da revista:
ENDOCRINOLOGIA
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Estados Unidos