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High concentrations of the melatonin metabolite, N1-acetyl-N2-formyl-5-methoxykynuramine, in cerebrospinal fluid of patients with meningitis: a possible immunomodulatory mechanism.
Silva, Sueli de Oliveira; Ximenes, Valdecir F; Livramento, José A; Catalani, Luiz H; Campa, Ana.
Afiliação
  • Silva SO; Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, SP, Brazil.
J Pineal Res ; 39(3): 302-6, 2005 Oct.
Article em En | MEDLINE | ID: mdl-16150112
We evaluated the presence of the melatonin metabolite N1-acetyl-N2-formyl-5-methoxykynuramine (AFMK), in cerebrospinal fluid (CSF) of patients with viral meningitis (n = 20) and control samples (n = 8) and correlate AFMK levels with inflammatory markers such as cellularity, protein, tumor necrosis factor (TNF)-alpha, interleukin (IL)-8 and IL-1beta levels. A portion of the CSF was extracted with dichloromethane (1:5) and analyzed by high-performance liquid chromatography (HPLC) under standardized conditions for AFMK. AFMK was detected in 16 of 20 CSF samples of patients with viral meningitis; the concentration of AFMK was found to be above the quantification limit (50 nmol/L) in six of these samples. AFMK was not detected in any of the eight control samples. The samples were classified into groups according to AFMK levels: undetectable (<10 nmol/L, group I), detectable but below the quantification limit (< 50 nmol/L, group II), and quantified (>50 nmol/L, group III). Group II presented the highest levels of proteins and IL-8, whereas group III showed the lowest levels of the inflammatory parameters. This study supports our hypothesis that inflammation favors the formation of AFMK and that this compound has immunomodulatory activity in vivo.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Adjuvantes Imunológicos / Cinuramina / Melatonina / Meningite Limite: Humans Idioma: En Revista: J Pineal Res Assunto da revista: ENDOCRINOLOGIA Ano de publicação: 2005 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Adjuvantes Imunológicos / Cinuramina / Melatonina / Meningite Limite: Humans Idioma: En Revista: J Pineal Res Assunto da revista: ENDOCRINOLOGIA Ano de publicação: 2005 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido