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Molecular typing of Mycobacterium bovis isolates from south-east Brazil by spoligotyping and RFLP.
Zanini, M S; Moreira, E C; Salas, C E; Lopes, M T P; Barouni, A S; Roxo, E; Telles, M A; Zumarraga, M J.
Afiliação
  • Zanini MS; Center of Agrarian Science, Federal University of Espírito Santo, PO Box 16, Alegre, ES, Brazil. zanini@cca.ufes.br
J Vet Med B Infect Dis Vet Public Health ; 52(3): 129-33, 2005 Apr.
Article em En | MEDLINE | ID: mdl-15876225
The identification of 163 strains of Mycobacterium bovis by polymerase chain reaction (PCR) and microbiological tests was carried out on 252 tuberculous-like lesions (TLLs) collected from slaughtered cattle in south-east Brazil. This study compared the usefulness of three genotyping techniques, IS6110-restriction fragment length polymorphism (RFLP), polymorphic guanine-cytosine-rich sequence (PGRS)-RFLP and direct repeat (DR)-spoligotyping, as applied to M. bovis isolates. Based on IS6110-RFLP genotyping we selected a group of 23 isolates containing more than one IS6110 copy, along with 16 samples containing one IS6110 copy from different geographical areas, evenly distributed among dairy (eight) and beef cattle (eight). These selected isolates were analysed by PGRS-RFLP and DR-spoligotyping genotyping. Dairy cattle (17%) display a higher frequency of multiple IS6110 copies than beef cattle (10%). A comparison between the genotype data obtained fails to show a correlation between the main clusters found by the three techniques. However, the clustering of each genotyping procedure revealed that the majority of strains are closely related. The RFLP-PGRS patterns showed a sizable group (20.5%) containing a 5.5 kb fragment and the predominant spoligotype is similar to that from the BCG vaccine strain. Unexpectedly, four strains (2.4%) showed drug resistance to 0.2 microg/ml isoniazid and 20 microg/ml ethionamide, but none of them was resistant to rifampicin or other antibiotics tested.
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tuberculose Bovina / Polimorfismo de Fragmento de Restrição / Técnicas de Tipagem Bacteriana / Mycobacterium bovis Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: J Vet Med B Infect Dis Vet Public Health Assunto da revista: MEDICINA VETERINARIA / SAUDE PUBLICA Ano de publicação: 2005 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha
Buscar no Google
Adicionar na Minha BVS
Imprimir
XML
PubMed Links

Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tuberculose Bovina / Polimorfismo de Fragmento de Restrição / Técnicas de Tipagem Bacteriana / Mycobacterium bovis Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: J Vet Med B Infect Dis Vet Public Health Assunto da revista: MEDICINA VETERINARIA / SAUDE PUBLICA Ano de publicação: 2005 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha