[Application of SensiScript enzyme to a RT-PCR system for the obtention of RNA of HIV-1 starting serum samples stored at -20 degrees C during a decade]. / Aplicación de la enzima SensiScript a un sistema RT-RCP para la obtención de ARN de VIH-1 a partir de muestras de suero almacenadas a -20 degrees C durante una década.
Rev Cubana Med Trop
; 55(3): 213-6, 2003.
Article
em Es
| MEDLINE
| ID: mdl-15849930
The efficiency of SensiScript reverse transciptase to obtain useful genetic material in the sequencing of the nucleic acid from HIV-1, starting from sera collected between 1989 and 1998 and kept at suboptimal temperatures, was proved. On using the SensiScript enzyme it was obtained an amplification of the RNA of the HIV-1 in 86.5 % of the studied samples, compared with 20 % on using the AMV-RT enzyme . No amplification was obtained in 13.5 % of the studied samples with any of the 2 enzymes used.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Viral
/
HIV-1
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Limite:
Humans
Idioma:
Es
Revista:
Rev Cubana Med Trop
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Cuba
País de publicação:
Cuba