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Improved anti-inflammatory and pharmacokinetic properties for superoxide dismutase by chemical glycosidation with carboxymethylchitin.
Valdivia, Aimara; Perez, Yunel; Dominguez, Amalia; Caballero, Julio; Gomez, Leissy; Schacht, Etienne H; Villalonga, Reynaldo.
Afiliação
  • Valdivia A; Enzyme Technology Group, Center for Biotechnological Studies, University of Matanzas, Autopista a Varadero Km 3 1/2, Matanzas, C.P. 44740, Cuba.
Macromol Biosci ; 5(2): 118-23, 2005 Feb 23.
Article em En | MEDLINE | ID: mdl-15729720
O-carboxymethylchitin (molecular weight = 1.07 x 10(5), degree of carboxymethylation = 80%, degree of N-acetylation = 91%) was chemically attached to superoxide dismutase by the formation of amide linkages through a carbodiimide catalyzed reaction. The glycosidated enzyme contained about 1.8 mole of polysaccharide per mole of protein and retained 57% of the initial catalytic activity. The anti-inflammatory activity of the enzyme was 2.4 times increased after conjugation with the polysaccharide. The modified superoxide dismutase preparation was remarkably more resistant to inactivation with H(2)O(2) and its plasma half-life time was prolonged from 4.8 min to 69 h.
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Superóxido Dismutase / Quitina / Anti-Inflamatórios Limite: Animals Idioma: En Revista: Macromol Biosci Assunto da revista: BIOQUIMICA Ano de publicação: 2005 Tipo de documento: Article País de afiliação: Cuba País de publicação: Alemanha
Buscar no Google
Adicionar na Minha BVS
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Superóxido Dismutase / Quitina / Anti-Inflamatórios Limite: Animals Idioma: En Revista: Macromol Biosci Assunto da revista: BIOQUIMICA Ano de publicação: 2005 Tipo de documento: Article País de afiliação: Cuba País de publicação: Alemanha