DNA-binding properties of cosmomycin D, an anthracycline with two trisaccharide chains.

Furlan, Renata L A; Watt, Stephen J; Garrido, Leandro M; Amarante-Mendes, Gustavo P; Nur-e-alam, Mohammad; Rohr, Jurgen; Braña, Alfredo; Mendez, Carmen; Salas, Jose A; Sheil, Margaret M; Beck, Jennifer L; Padilla, Gabriel

Furlan, Renata L A; Watt, Stephen J; Garrido, Leandro M; Amarante-Mendes, Gustavo P; Nur-e-alam, Mohammad; Rohr, Jurgen; Braña, Alfredo; Mendez, Carmen; Salas, Jose A; Sheil, Margaret M; Beck, Jennifer L; Padilla, Gabriel.

Afiliação

Furlan RL; Institute of Biomedical Sciences, University of Sao Paulo, SP, CEP 005508-900, Brazil.

J Antibiot (Tokyo) ; 57(10): 647-54, 2004 Oct.

Article em En | MEDLINE | ID: mdl-15638325

RESUMO

Cosmomycin D (CosD) is the major constituent fraction isolated from a culture of Streptomyces olindensis ICB20. The ability of this compound to intercalate with double-stranded DNA was studied by gel mobility shift assays and electrospray ionization mass spectrometry (ESI-MS). ESI-MS experiments showed that the complex of CosD with 16-mer double-stranded DNA was at least as stable as a complex of daunorubicin with the same DNA sequence. This is the first study showing DNA binding properties of an anthracycline containing a beta-rhodomycinone aglycone chromophore O-linked to two trisaccharide chains.

Assuntos

Antraciclinas/química; DNA/metabolismo; Antraciclinas/isolamento & purificação; Antraciclinas/metabolismo; Ensaio de Desvio de Mobilidade Eletroforética; Espectrometria de Massas por Ionização por Electrospray

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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA / Antraciclinas Idioma: En Revista: J Antibiot (Tokyo) Ano de publicação: 2004 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido

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