Mutacin production in Streptococcus mutans genotypes isolated from caries-affected and caries-free individuals.
Oral Microbiol Immunol
; 20(1): 20-4, 2005 Feb.
Article
em En
| MEDLINE
| ID: mdl-15612940
Relationships between genetic diversity and mutacin production in Streptococcus mutans were evaluated in 319 clinical isolates from eight caries-affected and eight caries-free individuals. The isolates were submitted to mutacin typing and AP-PCR (arbitrarily primed polymerase chain reaction) assay. The mutacin production was detected for 12 Streptococcus sp. indicator strains. Results showed significant variations in the mutacin production profiles and the inhibitory spectra of both groups. A possible association was seen between mutacin activity and the distinct patterns of Streptococcus sp. colonization in the two groups. Genotyping by AP-PCR using the primers OPA-02 and OPA-13 revealed 101 distinct genotypes against 48 phenotypes identified by mutacin typing. No correlation was observed between the inhibitory spectra of mutacin and genotypic similarities based on AP-PCR analyses. According to our results, strains of the same S. mutans genotype showed different mutacin profiles, suggesting a high degree of interstrain diversity. In conclusion, mutacin production seems to be of clinical importance in the colonization of S. mutans and is highly diversified in the S. mutans species.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Streptococcus mutans
/
Bacteriocinas
/
Cárie Dentária
Limite:
Adolescent
/
Adult
/
Humans
Idioma:
En
Revista:
Oral Microbiol Immunol
Assunto da revista:
ALERGIA E IMUNOLOGIA
/
MICROBIOLOGIA
/
ODONTOLOGIA
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Dinamarca