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High affinity leptin receptors are present in human mesenchymal stem cells (MSCs) derived from control and osteoporotic donors.
Hess, Rodrigo; Pino, Ana María; Ríos, Susana; Fernández, Mireya; Rodríguez, J Pablo.
Afiliação
  • Hess R; Laboratorio de Biología Celular, INTA, Universidad de Chile, Casilla 138-11, Santiago, Chile.
J Cell Biochem ; 94(1): 50-7, 2005 Jan 01.
Article em En | MEDLINE | ID: mdl-15517602
There are disparate observations on central and peripheral effects of leptin, but several studies consistently support its role as a link between fat and bone. Bone marrow stroma contains mesenchymal stem cells (MSCs), which differentiate into osteoblasts and adipocytes, among others. In this study we assessed the expression of leptin receptors protein in MSCs from control and osteoporotic postmenopausal donors and their change during osteogenic and adipogenic differentiation. Also, we assessed the effects of leptin on osteogenic and adipogenic differentiation of these cells. We demonstrated high affinity leptin binding (KD = 0.36 +/- 0.02 nM) in both types of cells. Binding was very low under basal, but increased significantly (2-3 times) through osteogenic and adipogenic differentiation. Osteoporotic MSCs showed lower leptin binding capacity than control cells at an early osteogenic and adipogenic differentiation time, which could restrict cell sensitivity to the protective action of leptin. In this regard, we observed that leptin significantly inhibited adipocyte differentiation in control but not in osteoporotic MSCs, while it exerted a low stimulatory effect on calcium deposition (10%-20%) in both types of MSCs cells. In summary, we report the presence of high affinity leptin receptors on control and osteoporotic MSCs, which were modified distinctly by osteogenic and adipogenic stimulation and a direct and distinct effect of leptin on both type of cells.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteoporose / Células-Tronco / Receptores de Superfície Celular / Mesoderma Tipo de estudo: Observational_studies Limite: Aged / Aged80 / Female / Humans Idioma: En Revista: J Cell Biochem Ano de publicação: 2005 Tipo de documento: Article País de afiliação: Chile País de publicação: Estados Unidos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteoporose / Células-Tronco / Receptores de Superfície Celular / Mesoderma Tipo de estudo: Observational_studies Limite: Aged / Aged80 / Female / Humans Idioma: En Revista: J Cell Biochem Ano de publicação: 2005 Tipo de documento: Article País de afiliação: Chile País de publicação: Estados Unidos