Characterization of SCAR markers of Eimeria spp. of domestic fowl and construction of a public relational database (The Eimeria SCARdb).
FEMS Microbiol Lett
; 238(1): 183-8, 2004 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-15336420
This study reports the development and characterization of 151 sequence characterized amplified region (SCAR) markers for the seven Eimeria species that infect the domestic fowl. From this set, 84 markers are species-specific and 67 present partial specificity. The complete nucleotide sequence was derived for all markers, revealing the presence of micro- and minisatellite repetitive units in 22 SCARs, with up to five distinct repeat units being observed per marker. Only 15 markers showed significant hits in similarity searches against public sequence databases, thus confirming their anonymous and non-coding character. Finally, a relational database of the markers (the Eimeria SCARdb) was developed and made available on the Internet, providing a valuable resource of SCAR markers that can be useful for molecular diagnosis, and also for epizootiological, genetic variability and genome mapping studies.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Aves Domésticas
/
Marcadores Genéticos
/
DNA de Protozoário
/
Bases de Dados de Ácidos Nucleicos
/
Eimeria
Limite:
Animals
Idioma:
En
Revista:
FEMS Microbiol Lett
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Reino Unido