Non-toxigenic pattern II and III Bacteroides fragilis strains: coexistence in the same host.
Res Microbiol
; 155(7): 522-4, 2004 Sep.
Article
em En
| MEDLINE
| ID: mdl-15313251
Diarrhoeic stool samples from 334 0-5-year-old children were analysed with respect to the incidence of Bacteroides fragilis as well as other enteropathogens. B. fragilis was recovered in 9.3% (31/334) of the samples, and 79 strains were examined for the presence of the bft gene or the BfPAI flanking region using polymerase chain reaction assays. No enterotoxigenic B. fragilis strains were detected. In 29% (9/31) of the samples the coexistence of both II and III non-toxigenic B. fragilis (NTBF) patterns could be seen. In 51.6% (16/31) of the samples there existed a pattern II NTBF only, and in 19.4% (6/31) only pattern III could be detected. Strains from the same patient representing different patterns were submitted to pulsed-field gel electrophoresis assays. Fingerprints obtained by this technique showed that there was strong heterogeneity among strains from different individuals. However, different patterns from the same individual shared 100% similarity.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bacteroides fragilis
/
Infecções por Bacteroides
/
Virulência
/
Regulação Bacteriana da Expressão Gênica
/
Enterotoxinas
/
Genes Bacterianos
Limite:
Child, preschool
/
Female
/
Humans
/
Infant
/
Male
/
Newborn
Idioma:
En
Revista:
Res Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
França