Isolation of two antigens from Trypanosoma evansi that are partially responsible for its cross-reactivity with Trypanosoma vivax.
Vet Parasitol
; 123(1-2): 67-81, 2004 Aug 13.
Article
em En
| MEDLINE
| ID: mdl-15265572
In Venezuela, two non-tsetse transmitted trypanosomes, Trypanosoma evansi and Trypanosoma vivax, are the major etiological agents of animal trypanosomosis. Rodents can be experimentally infected with T. evansi in order to obtain enough parasites to prepare antigens for serological tests. On the contrary, the production of T. vivax antigens is a limiting factor in most laboratories. Since T. evansi and T. vivax have exhibited a very high immunological cross-reactivity, we have focused on the identification of antigens from T. evansi responsible for this phenomenon. The predominant 64 kDa glycosylated cross-reacting antigen was recently purified from the TEVA1 T. evansi Venezuelan isolate [Parasitology 124 (2002) 287]. Here, we purified two additional cross-reacting antigens with molecular masses of approximately 51 and 68 kDa from the cytosolic fraction of the same T. evansi isolate, by sequential chromatography on DEAE-sepharose and sephacryl S-300. Sera obtained from animals infected with T. evansi or T. vivax recognized both purified proteins, suggesting their potential use as diagnostic reagents.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tripanossomíase Bovina
/
Doenças dos Bovinos
/
Trypanosoma vivax
/
Doenças dos Cavalos
/
Antígenos de Protozoários
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
País/Região como assunto:
America do sul
/
Venezuela
Idioma:
En
Revista:
Vet Parasitol
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Venezuela
País de publicação:
Holanda