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The anthracnose resistance locus Co-4 of common bean is located on chromosome 3 and contains putative disease resistance-related genes.
Melotto, M; Coelho, M F; Pedrosa-Harand, A; Kelly, J D; Camargo, L E A.
Afiliação
  • Melotto M; Departamento de Fitopatologia, Laboratório de Genética Molecular, ESALQ, Universidade de São Paulo, C.P. 9, 13418-900, Piracicaba, SP, Brazil. melottom@msu.edu
Theor Appl Genet ; 109(4): 690-9, 2004 Aug.
Article em En | MEDLINE | ID: mdl-15221144
The broadest based resistance to anthracnose of common bean ( Phaseolus vulgaris L.) is conferred by the Co-4 locus. We sequenced a bacterial artificial chromosome clone harboring part of the Co-4 locus of the bean genotype Sprite and assembled a single contig of 106.5 kb for functional annotation. This region contained five copies of the COK-4 gene that encodes for a serine threonine kinase protein previously mapped to the Co-4 locus and 19 novel genes with no similarity to any previously identified genes of common bean. Several putative genes of the Co-4 locus seemed to be expressed as they matched perfectly with bean expressed sequence tags. The expression of the COK-4 genes was assessed by reverse transcription (RT)-PCR, and a single 850-bp cDNA fragment was sequenced and compared with the genomic sequences of the COK-4 homologs. Although the COK-4 cDNA was isolated from a different bean cultivar, it showed high similarity (95%) to the exons of genes BA17 and BA21, suggesting that they were expressed. In a phylogenetic tree including all currently available Pto-like sequences from Phaseolus species, the COK-4 homologs formed a single cluster with the Pto gene, whereas two sequences from P. coccineus and all sequences of P. vulgaris formed two closely related clusters. The Co-4 locus was physically mapped to the short arm of bean chromosome 3, which corresponds to linkage group B8. This study represents a first step in gaining an understanding of the genomic organization of an anthracnose resistance locus of common bean and provides molecular data for comparative analysis with other plant species.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Filogenia / Doenças das Plantas / Ascomicetos / Phaseolus / Imunidade Inata Idioma: En Revista: Theor Appl Genet Ano de publicação: 2004 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Filogenia / Doenças das Plantas / Ascomicetos / Phaseolus / Imunidade Inata Idioma: En Revista: Theor Appl Genet Ano de publicação: 2004 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha