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Characterization and immunolocalization of an NTP diphosphohydrolase of Trypanosoma cruzi.
Fietto, Juliana L R; DeMarco, Ricardo; Nascimento, Ivan P; Castro, Ieso M; Carvalho, Técia M U; de Souza, Wanderley; Bahia, Maria T; Alves, Maria J M; Verjovski-Almeida, Sergio.
Afiliação
  • Fietto JL; Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, 35400-000 Ouro Preto, MG, Brazil.
Biochem Biophys Res Commun ; 316(2): 454-60, 2004 Apr 02.
Article em En | MEDLINE | ID: mdl-15020239
An ecto-NTP diphosphohydrolase (NTPDase) activity, insensitive to inhibitors of ATPases and phosphatases, was characterized on the surface of live Trypanosoma cruzi intact parasites. The enzyme exhibits broad substrate specificity, typical of NTPDases, and a high hydrolysis rate for GTP. A 2282 bp message encoding a full-length NTPDase was cloned by RT-PCR using epimastigote mRNA. A single protein was immunoprecipitated from [(35)S]methionine-labeled parasites using antibodies against Toxoplasma gondii NTPase I. This antibody localized an NTPDase on the external surface of all forms of T. cruzi, as seen by confocal immuno-fluorescence microscopy. The NTPDase could be part of the parasite's purine salvage pathway. Additionally, trypomastigotes (infective form) presented a 2:1 ATP/ADP hydrolysis ratio, while epimastigotes (non-infective form) presented a 1:1 ratio, suggesting a possible role for the NTPDase in the parasite's virulence mechanisms.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pirofosfatases / Trypanosoma cruzi Limite: Animals Idioma: En Revista: Biochem Biophys Res Commun Ano de publicação: 2004 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pirofosfatases / Trypanosoma cruzi Limite: Animals Idioma: En Revista: Biochem Biophys Res Commun Ano de publicação: 2004 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos