Kinetics and bioenergetics of Spirulina platensis cultivation by fed-batch addition of urea as nitrogen source.
Appl Biochem Biotechnol
; 112(3): 143-50, 2004 Mar.
Article
em En
| MEDLINE
| ID: mdl-15007182
The cyanobacterium Spirulina platensis was cultivated in bench-scale miniponds on bicarbonate/carbonate solutions using urea as nitrogen source. To minimize limitation and inhibition phenomena, urea was supplied semicontinuously using exponentially increasing feeding rates. The average growth rates obtained alternately varying the total mass of urea added per unit reactor volume (275 < mT < 725 mg/L) and the total feeding time (9 < tT < 15 d) clearly evidenced nitrogen limitation for mT< 500 mg/L and excess nitrogen inhibition above this threshold. The time behavior of the specific growth rate at variable urea feeding patterns allowed estimation of the time-dependent Gibbs energy dissipation for cell growth under the actual depletion conditions of fed-batch cultivations. Comparison of the yield of growth on Gibbs energy obtained using either urea or KNO3 pointed to the preference of S. platensis for the former nitrogen source, likely owing to more favorable bioenergetic conditions.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ureia
/
Cianobactérias
/
Metabolismo Energético
/
Nitrogênio
Idioma:
En
Revista:
Appl Biochem Biotechnol
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Estados Unidos