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Development and validation of a reversed-phase liquid chromatographic method for analysis of D4T (Stavudine) in rat plasma.
Contreras, J; González, H Ma; Menéndez, R; López, M.
Afiliação
  • Contreras J; Department of Analytical Chemistry, HPLC and Pharmacokinetics Laboratory, National Center for Scientific Research, PO Box 6412/6414, Havana, Cuba. contreras@diramic.cneuro.edu.cu
J Chromatogr B Analyt Technol Biomed Life Sci ; 801(2): 199-203, 2004 Mar 05.
Article em En | MEDLINE | ID: mdl-14751787
An improved and simplified high-performance liquid chromatographic (HPLC) method at UV detection 265 nm is presented for the determination of d4T in rat plasma. The mobile phase consists of methanol-distilled water-acetic acid in the 23:77:0.2 (v/v) ratio. Quantification is achieved by the peak-area ratio method with reference to the internal standard. This paper presents linearity, accuracy, precision, limit of quantification and limit of detection, specificity-selectivity and sample stability data. Based on the intra and inter-day validation, all coefficients of variation (CV) were found less than 15%. The assay is sufficiently rapid and sensitive and was applied in a pharmacokinetic study in rats.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cromatografia Líquida de Alta Pressão / Estavudina / Fármacos Anti-HIV Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2004 Tipo de documento: Article País de afiliação: Cuba País de publicação: Holanda
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cromatografia Líquida de Alta Pressão / Estavudina / Fármacos Anti-HIV Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2004 Tipo de documento: Article País de afiliação: Cuba País de publicação: Holanda