Development and validation of a reversed-phase liquid chromatographic method for analysis of D4T (Stavudine) in rat plasma.
J Chromatogr B Analyt Technol Biomed Life Sci
; 801(2): 199-203, 2004 Mar 05.
Article
em En
| MEDLINE
| ID: mdl-14751787
An improved and simplified high-performance liquid chromatographic (HPLC) method at UV detection 265 nm is presented for the determination of d4T in rat plasma. The mobile phase consists of methanol-distilled water-acetic acid in the 23:77:0.2 (v/v) ratio. Quantification is achieved by the peak-area ratio method with reference to the internal standard. This paper presents linearity, accuracy, precision, limit of quantification and limit of detection, specificity-selectivity and sample stability data. Based on the intra and inter-day validation, all coefficients of variation (CV) were found less than 15%. The assay is sufficiently rapid and sensitive and was applied in a pharmacokinetic study in rats.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromatografia Líquida de Alta Pressão
/
Estavudina
/
Fármacos Anti-HIV
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Chromatogr B Analyt Technol Biomed Life Sci
Assunto da revista:
ENGENHARIA BIOMEDICA
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Cuba
País de publicação:
Holanda