Biodegradation of alachlor by soil streptomycetes.
Appl Microbiol Biotechnol
; 64(5): 712-7, 2004 Jun.
Article
em En
| MEDLINE
| ID: mdl-14727088
Streptomycetes resistant to the herbicide alachlor [2-chloro-2',6'-diethyl- N-(methoxymethyl) acetanilide] were used in degradation assays to characterize the products of alachlor biodegradation. Of six strains tested, Streptomyces sp. LS166, LS177, and LS182 were able to grow at an alachlor concentration of 144 mg l(-1) and degraded approximately 60-75% of the alachlor in 14 days, as evaluated by high performance liquid chromatography. The alachlor biodegradation products were identified by gas chromatography-mass spectrometry based on mass spectral data and fragmentation patterns. All compounds detected in these assays were similar for all streptomycetes strains tested, and involved dechlorination with subsequent N-dealkylation and cyclization of the remaining N-substituent with one of the ethyl groups to produce indole and quinoline derivatives. The enzymatic pathway used by Streptomyces sp. LS182 did not generate DEA (2',6'-diethylaniline), a carcinogenic derivative of alachlor reported in other studies. Given the high degradation rates observed here, the Streptomyces strains tested may be useful in the degradation/detoxification processes of alachlor.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Microbiologia do Solo
/
Streptomycetaceae
/
Poluentes Químicos da Água
/
Herbicidas
/
Acetamidas
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Appl Microbiol Biotechnol
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Alemanha