Crystallization and preliminary X-ray diffraction analysis of levansucrase (LsdA) from Gluconacetobacter diazotrophicus SRT4.
Acta Crystallogr D Biol Crystallogr
; 60(Pt 1): 181-3, 2004 Jan.
Article
em En
| MEDLINE
| ID: mdl-14684923
The endophytic bacterium Gluconacetobacter diazotrophicus SRT4 secretes a constitutively expressed levansucrase (LsdA; EC 2.4.1.10), which converts sucrose to fructo-oligosaccharides and levan. Fully active LsdA was purified to high homogeneity by non-denaturing reversed-phase HPLC and was crystallized at room temperature by the hanging-drop vapour-diffusion method using ammonium sulfate and ethanol as precipitants. The crystals are extremely sensitive, but native data have been collected to 2.5 A under cryogenic conditions using synchrotron radiation. LsdA crystals belong to the orthorhombic space group P22(1)2(1) or P2(1)2(1)2, with unit-cell parameters a = 53.80, b = 119.39, c = 215.10 A.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Gluconacetobacter
/
Hexosiltransferases
Idioma:
En
Revista:
Acta Crystallogr D Biol Crystallogr
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Cuba
País de publicação:
Estados Unidos