Crystallization and preliminary X-ray diffraction analysis of levansucrase (LsdA) from Gluconacetobacter diazotrophicus SRT4.

Martínez-Fleites, Carlos; Tarbouriech, Nicolas; Ortiz-Lombardia, Miguel; Taylor, Edward; Rodríguez, Armando; Ramírez, Ricardo; Hernández, Lázaro; Davies, Gideon J

Martínez-Fleites, Carlos; Tarbouriech, Nicolas; Ortiz-Lombardia, Miguel; Taylor, Edward; Rodríguez, Armando; Ramírez, Ricardo; Hernández, Lázaro; Davies, Gideon J.

Afiliação

Martínez-Fleites C; Physical Chemistry Division, Center for Genetic Engineering and Biotechnology, PO Box 6162, Havana 10600, Cuba.

Acta Crystallogr D Biol Crystallogr ; 60(Pt 1): 181-3, 2004 Jan.

Article em En | MEDLINE | ID: mdl-14684923

RESUMO

The endophytic bacterium Gluconacetobacter diazotrophicus SRT4 secretes a constitutively expressed levansucrase (LsdA; EC 2.4.1.10), which converts sucrose to fructo-oligosaccharides and levan. Fully active LsdA was purified to high homogeneity by non-denaturing reversed-phase HPLC and was crystallized at room temperature by the hanging-drop vapour-diffusion method using ammonium sulfate and ethanol as precipitants. The crystals are extremely sensitive, but native data have been collected to 2.5 A under cryogenic conditions using synchrotron radiation. LsdA crystals belong to the orthorhombic space group P22(1)2(1) or P2(1)2(1)2, with unit-cell parameters a = 53.80, b = 119.39, c = 215.10 A.

Assuntos

Gluconacetobacter/enzimologia; Hexosiltransferases/química; Cristalização; Cristalografia por Raios X

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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Gluconacetobacter / Hexosiltransferases Idioma: En Revista: Acta Crystallogr D Biol Crystallogr Ano de publicação: 2004 Tipo de documento: Article País de afiliação: Cuba País de publicação: Estados Unidos

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