Determination of diclofenac sodium in eagle's minimum essential medium with Earle's balanced salt solution.
J AOAC Int
; 86(4): 681-4, 2003.
Article
em En
| MEDLINE
| ID: mdl-14509424
A validated method was developed for determination of diclofenac sodium, considered a model hydrophilic drug for in vitro permeation studies, in Eagle's Minimum Essential Medium (MEM) with Earle's balanced salt solution. Liquid chromatography was used to determine diclofenac sodium. This method was developed with a reversed-phase column Supercosil LC 18, DB 25 cm x 4.5 mm; the mobile phase was methanol with 3% (v/v) acetic acid-Milli-Q water (74 + 26), and detection was at 283 nm. The detection and quantitation limits were 2.41 x 10(-8) and 3.31 x 10(-5) microg/microL, respectively. The accuracy within-day (n = 3) and day-to-day (n = 7) was 98.83%; the mean variation coefficient for inter- (n = 7) and intraday precision (n = 3) was 12.20%, thus, not exceeding 15%. This method can be used as an analytical procedure for the determination of diclofenac sodium in MEM for in vitro permeation studies.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Diclofenaco
/
Cromatografia Líquida de Alta Pressão
/
Meios de Cultura
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Idioma:
En
Revista:
J AOAC Int
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Reino Unido