Insulin and lipogenesis in rat adipocytes. I. Effect of insulin incubation on lipid synthesis by isolated rat adipocytes.

Morales, L M; Campos, G; Ryder, E; Casanova, A

Morales, L M; Campos, G; Ryder, E; Casanova, A.

Afiliação

Morales LM; Facultad de Ciencias, Universidad del Zulia, Maracaibo, Venezuela.

Biochem Biophys Res Commun ; 188(2): 807-12, 1992 Oct 30.

Article em En | MEDLINE | ID: mdl-1445324

RESUMO

To assess the effect of insulin on lipid synthesis in isolated rat adipocytes, cells were preincubated for 3 h with high concentrations (16.6 nM) of the hormone and lipogenesis measured through 14C-acetate incorporation into lipids, analyzing at the same time the activity of some lipogenic enzymes. It was found that insulin induced not only a decrease in the number of insulin receptors but a 30% loss in basal and insulin-stimulated acetate incorporation into total lipids as well as a decrease in the activities of enzymes related to the novo fatty acid synthesis pathway as malic enzyme and glucose-6-phosphate dehydrogenase.

Assuntos

Tecido Adiposo/metabolismo; Insulina/farmacologia; Lipídeos/biossíntese; Acetatos/metabolismo; Tecido Adiposo/efeitos dos fármacos; Animais; Radioisótopos de Carbono; Células Cultivadas; DNA/metabolismo; Glucosefosfato Desidrogenase/metabolismo; Cinética; Malato Desidrogenase/metabolismo; Masculino; Complexos Multienzimáticos/metabolismo; Oxo-Ácido-Liases/metabolismo; Ratos; Ratos Sprague-Dawley; Receptor de Insulina/efeitos dos fármacos; Receptor de Insulina/metabolismo

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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tecido Adiposo / Insulina / Lipídeos Limite: Animals Idioma: En Revista: Biochem Biophys Res Commun Ano de publicação: 1992 Tipo de documento: Article País de afiliação: Venezuela País de publicação: Estados Unidos

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