Characterization of the colorectal antigen IOR-C2.
Hybridoma
; 11(2): 245-56, 1992 Apr.
Article
em En
| MEDLINE
| ID: mdl-1376720
A colorectal antigen (IOR-C2) was characterized by a monoclonal antibody produced against the colon cancer cell line SW1116. By immunohistochemical staining the antigen was abundant and strongly expressed in epithelium of normal colon whereas colorectal carcinomas showed a more variable and heterogenous reactivity to the antibody (IOR-C2). Radioimmunoprecipitates of SW1116 cell homogenates showed a 160-200 kD band in SDS gels. Physicochemical characterization indicate that at least two IOR-C2 reactive sites are present on the antigen tested and that it is mainly an 0-linked glycoprotein carbohydrate chain which can also be N-linked to the protein. The expression of IOR-C2 mimics that of the colon associated antigen (CAA) and NCC-CO-450 antigen but is distinct from these with regard to its expression in carcinomas as well as its physicochemical characteristics.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Colorretais
/
Antígenos de Neoplasias
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Hybridoma
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
Cuba
País de publicação:
Estados Unidos