Presence and subcellular localization of tyrosine aminotransferase and p-hydroxyphenyllactate dehydrogenase in epimastigotes of Trypanosoma cruzi.

Nowicki, C; Montemartini, M; Duschak, V; Santomé, J A; Cazzulo, J J

Nowicki, C; Montemartini, M; Duschak, V; Santomé, J A; Cazzulo, J J.

Afiliação

Nowicki C; IQUIFIB, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.

FEMS Microbiol Lett ; 71(2): 119-24, 1992 Apr 15.

Article em En | MEDLINE | ID: mdl-1351016

RESUMO

Cell-free extracts of epimastigotes of Trypanosoma cruzi contain tyrosine aminotransferase (TAT) and p-hydroxyphenyllactate dehydrogenase (pHPLDH). The TAT activity could be separated from aspartate aminotransferase (ASAT) by polyacrylamide gel electrophoresis or DEAE-cellulose chromatography; the latter procedure also allowed complete separation of pHPLDH. The subcellular localization of both T. cruzi enzymes, as determined by digitonin extraction, subcellular fractionation by differential centrifugation, and isopycnic ultracentrifugation in sucrose gradients, was mainly cytosolic, with low mitochondrial activities.

Assuntos

Oxirredutases/isolamento & purificação; Fenilpropionatos/metabolismo; Trypanosoma cruzi/enzimologia; Tirosina Transaminase/isolamento & purificação; Animais; Ciclo Celular; Frações Subcelulares/enzimologia; Trypanosoma cruzi/ultraestrutura

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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oxirredutases / Fenilpropionatos / Trypanosoma cruzi / Tirosina Transaminase Limite: Animals Idioma: En Revista: FEMS Microbiol Lett Ano de publicação: 1992 Tipo de documento: Article País de afiliação: Argentina País de publicação: Reino Unido

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