A rapid, reliable method of evaluating growth and viability of intraerythrocytic protozoan hemoparasites using fluorescence flow cytometry.
Mem Inst Oswaldo Cruz
; 87 Suppl 3: 235-9, 1992.
Article
em En
| MEDLINE
| ID: mdl-1343696
Fluorescence flow cytometry was employed to assess the potential of a vital dye, hydroethidine, for use in the detection and monitoring of the viability of hemoparasites in infected erythrocytes, using Babesia bovis as a model parasite. The studies demonstrated that hydroethidine is taken up by B. bovis and metabolically converted to the DNA binding fluorochrome, ethidium. Following uptake of the dye, erythrocytes containing viable parasites were readily distinguished and quantitated. Timed studies with the parasiticidal drug, Ganaseg, showed that it is possible to use the fluorochrome assay to monitor the effects of the drug on the rate of replication and viability of B. bovis in culture. The assay provides a rapid method for evaluation of the in vitro effect of drugs on hemoparasites and for analysis of the effect of various components of the immune response, such as lymphokines, monocyte products, antibodies, and effector cells (T, NK, LAK, ADCC) on the growth and viability of intraerythrocytic parasites.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fenantridinas
/
Babesia bovis
/
Eritrócitos
/
Citometria de Fluxo
Limite:
Animals
Idioma:
En
Revista:
Mem Inst Oswaldo Cruz
Ano de publicação:
1992
Tipo de documento:
Article
País de publicação:
Brasil