Protective effect of prolactin and placental lactogen on NO-induced Nb2 lymphoma cell apoptosis.
Arch Biochem Biophys
; 416(2): 249-56, 2003 Aug 15.
Article
em En
| MEDLINE
| ID: mdl-12893303
Nitric oxide (NO) is an important modulator involved in immune regulation. Here, we describe conditions under which NO-donors induce apoptosis on Nb2 lymphoma cells, as evidenced by decreased cell viability and increased hypodiploid DNA content determined by flow cytometry. In addition, DNA fragmentation typical of apoptosis was shown by agarose gel electrophoresis. This apoptosis was accompanied by a significant increase of caspase-3-like enzymatic activity. Both ovine prolactin (oPRL) and ovine placental lactogen (oPL) exerted a protective effect on the NO-donor-induced apoptosis. Furthermore, dexamethasone (Dex)-induced cell death was also associated with caspase-3-like activity and oPL had the same potency as oPRL in its protective effect on Dex-induced apoptosis of Nb2 cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Lactogênio Placentário
/
Prolactina
/
Apoptose
/
Linfoma
/
Óxido Nítrico
Limite:
Animals
Idioma:
En
Revista:
Arch Biochem Biophys
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Argentina
País de publicação:
Estados Unidos