A chloride-permeable channel from Phaseolus vulgaris roots incorporated into planar lipid bilayers.

Balleza, Daniel; Quinto, Carmen; Sánchez, Federico; Gómez-Lagunas, Froylan

Balleza, Daniel; Quinto, Carmen; Sánchez, Federico; Gómez-Lagunas, Froylan.

Afiliação

Balleza D; Departamento de Biologi;a Molecular de Plantas, Instituto de Biotecnologi;a, UNAM, Cuernavaca Morelos 62210, Mexico.

Biochem Biophys Res Commun ; 307(1): 114-8, 2003 Jul 18.

Article em En | MEDLINE | ID: mdl-12849989

RESUMO

Ion channels are key participants in physiological processes of plant cells. Here, we report the first characterization of a high conductance, Cl(-)-permeable channel, present in enriched fractions of plasma membranes of bean root cells. The Cl(-) channel was incorporated into planar lipid bilayers and its activity was recorded under voltage clamp conditions. The channel is voltage-dependent, excludes the passage of cations (K(+), Na(+), and Ca(2+)), and is inhibited by micromolar concentrations of Zn(2+). The Cl(-) conductance here characterized represents a previously undescribed channel of plant cells.

Assuntos

Canais de Cloreto/metabolismo; Bicamadas Lipídicas/metabolismo; Phaseolus/metabolismo; Proteínas de Plantas/metabolismo; Raízes de Plantas/metabolismo; Membrana Celular/metabolismo; Ativação do Canal Iônico; Técnicas de Patch-Clamp; Phaseolus/anatomia & histologia; Zinco/metabolismo

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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Canais de Cloreto / Raízes de Plantas / Phaseolus / Bicamadas Lipídicas Idioma: En Revista: Biochem Biophys Res Commun Ano de publicação: 2003 Tipo de documento: Article País de afiliação: México País de publicação: Estados Unidos

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