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An experimental model of hemolysis-induced acute pancreatitis.
Saruc, M; Yuceyar, H; Turkel, N; Ozutemiz, O; Tuzcuoglu, I; Yuce, G; Huseyinov, A.
Afiliação
  • Saruc M; Department of Gastroenterology, Celal Bayar University, Manisa, Turkey. msaruc@hotmail.com
Braz J Med Biol Res ; 36(7): 879-86, 2003 Jul.
Article em En | MEDLINE | ID: mdl-12845374
The literature indicates that acute pancreatitis is a complication of massive hemolysis with a prevalence of about 20%. We describe an experimental model of hemolysis-induced acute pancreatitis. Hemolytic anemia was induced in rats by a single ip injection of 60 mg/kg of 20 mg/ml acetylphenylhydrazine (APH) in 20% (v/v) ethanol on the first experimental day (day 0). One hundred and fifty Wistar albino rats weighing 180-200 g were divided into three groups of 50 animals each: groups 1, 2 and 3 were injected ip with APH, 20% ethanol, and physiological saline, respectively. Ten rats from each group were sacrificed on study days 1, 2, 3, 4 and 5. Serum amylase, lipase levels and pancreatic tissue tumor necrosis factor-alpha (TNF-alpha) and platelet-activating factor (PAF) contents were determined and a histological examination of the pancreas was performed. No hemolysis or pancreatitis was observed in any of the rats in groups 2 and 3. In group 1, massive hemolysis was observed in 35 (70%) of 50 rats, moderate hemolysis in seven (14%), and no hemolysis in eight (16%). Thirty-three of 35 (94.2%) rats with massive hemolysis had hyperamylasemia, and 29 of these rats (82.8%) had histologically proven pancreatitis. The most severe pancreatitis occurred on day 3, as demonstrated by histology. Tissue TNF-alpha and PAF levels were statistically higher in group 1 than in groups 2 and 3. Acute massive hemolysis induced acute pancreatitis, as indicated by histology, in almost 80% of cases. Hemolysis may induce acute pancreatitis by triggering the release of proinflammatory and immunoregulatory cytokines.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pancreatite / Hemólise / Anemia Hemolítica Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Animals Idioma: En Revista: Braz J Med Biol Res Ano de publicação: 2003 Tipo de documento: Article País de afiliação: Turquia País de publicação: Brasil
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pancreatite / Hemólise / Anemia Hemolítica Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Animals Idioma: En Revista: Braz J Med Biol Res Ano de publicação: 2003 Tipo de documento: Article País de afiliação: Turquia País de publicação: Brasil