Polymorphism at position -174 of IL-6 gene is associated with susceptibility to chronic periodontitis in a Caucasian Brazilian population.

Trevilatto, P C; Scarel-Caminaga, R M; de Brito, R B; de Souza, A P; Line, S R P

Trevilatto, P C; Scarel-Caminaga, R M; de Brito, R B; de Souza, A P; Line, S R P.

Afiliação

Trevilatto PC; Department of Morphology, Dental School of Piracicaba, State University of Campinas, SP, Brazil.

J Clin Periodontol ; 30(5): 438-42, 2003 May.

Article em En | MEDLINE | ID: mdl-12716337

RESUMO

BACKGROUND: Interleukin-6 (IL-6) is a multifunctional cytokine that mediates inflammatory tissue destruction. A G to C substitution at position -174 in the promoter of IL-6 gene reduces in vitro transcription of IL-6. This polymorphism has been associated with inflammatory diseases like chronic arthritis. OBJECTIVE: The aim of this study was to investigate the association between the IL-6-174 polymorphism and susceptibility to chronic periodontitis in Brazilians. MATERIAL AND METHODS: Eighty-four nonsmoking subjects over 25 years (mean age 42.4) were divided according to the severity level of periodontal disease: 36 healthy individuals (control group), 24 subjects with moderate and 24 with severe periodontitis. Genomic DNA was obtained from epithelial cells through a mouthwash with 3% glucose and scraping of oral mucosa. The samples were analyzed for IL-6-174 polymorphism using PCR-RFLP. The significance of the differences in the frequencies of the polymorphism in the control and groups with periodontitis was assessed by chi2 test (p<0.05). RESULTS: Differences were found between control and groups with periodontitis in the genotype (p=0.0036, OR=3.0) and in the allele (p=0.0838, OR=1.9) frequencies. CONCLUSION: We concluded that the IL-6-174 polymorphism is associated with susceptibility to chronic periodontitis in the population studied.

Assuntos

Predisposição Genética para Doença/genética; Interleucina-6/genética; Periodontite/genética; Polimorfismo Genético/genética; População Branca/genética; Adulto; Alelos; Sequência de Bases; Brasil; Distribuição de Qui-Quadrado; Doença Crônica; Citosina; Feminino; Frequência do Gene/genética; Genótipo; Guanina; Humanos; Mediadores da Inflamação/imunologia; Masculino; Periodontite/classificação; Periodontite/imunologia; Regiões Promotoras Genéticas/genética; Transcrição Gênica/genética

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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Periodontite / Polimorfismo Genético / Interleucina-6 / Predisposição Genética para Doença / População Branca Tipo de estudo: Risk_factors_studies Limite: Adult / Female / Humans / Male País/Região como assunto: America do sul / Brasil Idioma: En Revista: J Clin Periodontol Ano de publicação: 2003 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos

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