In vitro culture and biochemical characterization of six trypanosome isolates from Peru and Brazil.
Exp Parasitol
; 102(1): 23-9, 2002 Sep.
Article
em En
| MEDLINE
| ID: mdl-12615163
Six trypanosomatids isolated from different geographical areas from South America (Peru and Brazil) and different vectors and reservoir hosts (the triatomine Panstrongylus chinai [TP1], Triatoma infestans [TP2], Rhodnius ecuadorensis [TP3], R. prolixus [TB1], Didelphys marsupialis [TB2]), and one from a human asymptomatic patient [TB3], were characterized using lectin agglutination, isoenzyme profile, in vitro culture final metabolite patterns, and compared with a reference strain (Trypanosoma cruzi, Maracay strain [TC]). The different isolates were cultured in vitro in Grace's medium supplemented with 10% inactivated bovine foetal serum. According to our results and the statistical study, the isolate obtained from R. ecuadorensis should be designed as a Trypanosoma rangeli sp., showing all other isolates strong similarities to T. cruzi. Between them, two clusters could be identified, strongly correlating with the geographical origin. Cluster I grouped isolates from Peru and T. cruzi reference strain, and cluster II grouped the three Brazilian isolates.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Trypanosoma
/
Trypanosoma cruzi
Limite:
Animals
/
Humans
País/Região como assunto:
America do sul
/
Brasil
/
Peru
Idioma:
En
Revista:
Exp Parasitol
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Espanha
País de publicação:
Estados Unidos