[Comparison of three neuro-tracing techniques for identification of the sciatic spinal nerve origin in mice]. / Comparación de tres técnicas de trazado retrógrado para la identificación del origen espinal del nervio ciático en ratón.
Biomedica
; 22(4): 529-34, 2002 Dec.
Article
em Es
| MEDLINE
| ID: mdl-12596451
To identify sensory and motor neurons associated with the sciatic nerve in adult mice, three methods for applying fluorescent tracers (Fluorogold and Dil) were investigated: direct application, intraneural injection and impregnation of a sectioned nerve in a silicone chamber. Most accurate localization of the neurons on the dorsal root ganglia and spinal cord was accomplished by introducing the proximal stump of a transected sciatic nerve into a silicone chamber, filled with tracers and then decalcifying the tissue. Fluorogold was an effective tracing agent, in contrast to Dil, which was not. In addition to associations with cephalic ganglia L4, L5 and L6, as seen in rats, contributory neurons to the sciatic nerve were located in other ganglia in the mouse. These findings show that the silicone chamber-tissue decalcification technique is a viable tool for obtaining comparative neuroanatomical information in the mouse model.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Nervo Isquiático
/
Estilbamidinas
/
Carbocianinas
/
Corantes Fluorescentes
/
Gânglios Espinais
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
Es
Revista:
Biomedica
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Colômbia
País de publicação:
Colômbia