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Lipopolysaccharide-cell interaction and induced cellular activation in whole blood of septic patients.
Salomao, Reinaldo; Brunialti, Milena K C; Kallás, Esper G; Martins, Paulo S; Rigato, Otelo; Freudenberg, Marina.
Afiliação
  • Salomao R; Division of Infectious Diseases, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil. rsalomao-dipa@pesquisa.epm.br
J Endotoxin Res ; 8(5): 371-9, 2002.
Article em En | MEDLINE | ID: mdl-12537696
We used biotinylated LPS (LPSb) and flow cytometry to study LPS-monocyte interaction and LPS-induced cellular activation in whole blood from septic patients (SP). Expression of surface activation markers was evaluated on monocytes (HLA-DR) and T lymphocytes (CD69 and CD95), and intracellular TNF-alpha on monocytes. Saturating curve and kinetics of LPSb detection on monocytes were similar in SP and healthy volunteers (HV). LPSb bound to monocytes was detected after 5 min of incubation in both groups, with a more pronounced decay in SP. Monocytes from SP had a lower expression of HLA-DR as compared to HV, both constitutive and upon LPS stimulation. The proportion of monocytes producing TNF-alpha after LPS stimulus was higher in HV than SP (mean +/- SD = 25.2 +/- 14.2% and 2.2 +/- 2.6%, respectively, P < 0.001). LPS-induced CD69 on T CD8+ and CD8- lymphocytes was similar for patients and controls. Expression of CD95 on T lymphocytes was higher in SP as compared to HV on T CD8+ cells (GMFI, mean +/- SD = 22.3 +/- 14.6 and 8.6 +/- 5.0, respectively, P = 0.01) and CD8- cells (GMFI, mean +/- SD = 28.3 +/- 7.7 and 14 +/- 4.3 respectively, P < 0.001). Thus, monocytes and lymphocytes seem to respond differently to LPS in septic patients. Monocyte hyporesponsiveness appears not to be related to a decreased binding capacity of LPS, but rather to an impaired signal transduction.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ativação Linfocitária / Monócitos / Linfócitos T / Lipopolissacarídeos / Sepse Tipo de estudo: Observational_studies Limite: Adolescent / Adult / Humans / Male Idioma: En Revista: J Endotoxin Res Assunto da revista: ANGIOLOGIA / BACTERIOLOGIA Ano de publicação: 2002 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ativação Linfocitária / Monócitos / Linfócitos T / Lipopolissacarídeos / Sepse Tipo de estudo: Observational_studies Limite: Adolescent / Adult / Humans / Male Idioma: En Revista: J Endotoxin Res Assunto da revista: ANGIOLOGIA / BACTERIOLOGIA Ano de publicação: 2002 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos