A custom-built controlled-rate freezer for small sample cryopreservation studies.

Medrano, A; Anderson, J; Millar, J D; Holt, W V; Watson, P F

Medrano, A; Anderson, J; Millar, J D; Holt, W V; Watson, P F.

Afiliação

Medrano A; Department of Animal Sciences, Facultad de Estudios Superiores - Cuautitlan, National Autonomous University of Mexico 54740 Cuautitlan Izcalli, Mexico. amedrano@servidor.unam.mx

Cryo Letters ; 23(6): 397-404, 2002.

Article em En | MEDLINE | ID: mdl-12522510

RESUMO

A custom-built controlled-rate freezing machine is described. It operates by automatically raising and lowering the sample carrier in a static column of liquid nitrogen vapour. It is controlled by a computer-assisted thermocouple feedback system that operates a stepper motor driving the sample carrier. The cooling protocol is divided into three phases: cooling from +5 to -5 C, initiation of ice nucleation, and cooling from -5 to -80 C. Experiments are described to validate the device over a range of different cooling rates. A freezing protocol is established to cool samples in plastic straws over a range of rates up to 80 C/min, with rapid and consistent absorption of the latent heat.

Assuntos

Criopreservação/instrumentação; Animais; Desenho de Equipamento; Congelamento; Masculino; Preservação do Sêmen/instrumentação; Suínos

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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Criopreservação Limite: Animals Idioma: En Revista: Cryo Letters Assunto da revista: BIOLOGIA / QUIMICA Ano de publicação: 2002 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido

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