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Progesterone upregulates GATA-1 on erythroid progenitors cells in liquid culture.
da Silva Santos Duarte, Adriana; Sales, Tereza Sueko Ide; Mengel, José O; Costa, Fernando Ferreira; Saad, Sara Teresinha Olalla.
Afiliação
  • da Silva Santos Duarte A; Hematology and Hemotherapy Centre, State University of Campinas, Campinas, SP, Brazil.
Blood Cells Mol Dis ; 29(2): 213-24, 2002.
Article em En | MEDLINE | ID: mdl-12490288
Steroids hormones modify the hematological features of homozygous sickle cell disease, including the levels of fetal hemoglobin. We used semi-quantitative RT-PCR analysis of GATA-1, GATA-2, NF-E2, and gamma-globin mRNA levels in a two-phase liquid culture system of human adult erythroid cells in order to assay the effect of progesterone upon gene expression. The levels of expression of GATA-1 and gamma-globin mRNA were significantly increased in cells treated with progesterone compared to untreated cells (1.7- to 2.0-fold). Progesterone treatment did not produce any stimulatory effect upon GATA-2 and NF-E2 mRNA expression. Differences in the synthesis of HbF protein could not be detected by flow cytometry, although we observed a small difference in mean intensity fluorescence between cells treated and cells untreated with progesterone on days 7 and 9. Using anti-transferrin receptor and anti-glycophorin A antibodies, we verified that addition of progesterone did not cause any change in erythroid proliferation and differentiation. In conclusion, it is possible that the increased expression of gamma-globin mRNA after progesterone treatment observed in this study may be related to the increased GATA-1 mRNA expression. Interactions of the steroid receptors with the basal transcriptional machinery and with transcription factors might mediate their transcriptional effects.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Progesterona / Fatores de Transcrição / Células Precursoras Eritroides / Regulação para Cima / Proteínas de Ligação a DNA Limite: Humans Idioma: En Revista: Blood Cells Mol Dis Assunto da revista: HEMATOLOGIA Ano de publicação: 2002 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Progesterona / Fatores de Transcrição / Células Precursoras Eritroides / Regulação para Cima / Proteínas de Ligação a DNA Limite: Humans Idioma: En Revista: Blood Cells Mol Dis Assunto da revista: HEMATOLOGIA Ano de publicação: 2002 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos